期刊
ANALYTICAL BIOCHEMISTRY
卷 377, 期 1, 页码 33-39出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.03.007
关键词
nanocalorimetry; enzyme assay; binding assay; label-free assay
资金
- NIBIB NIH HHS [R01 EB009191] Funding Source: Medline
- NIGMS NIH HHS [1R01GM077435-01, R01 GM077435, R01 GM077435-02] Funding Source: Medline
- PHS HHS [266200400058C] Funding Source: Medline
Enthalpy arrays enable label-free, solution-based calorimetric detection of molecular interactions in a 96-detector array format. The combination of the small size of the detectors and the ability to perform measurements in parallel results in a significant reduction of sample volume and measurement time compared with conventional calorimetry. We have made significant improvements in the technology by reducing the temperature noise of the detectors and improving the fabrication materials and methods. In combination with an automated measurement system, the advances in device performance and data analysis have allowed us to develop basic enzyme assays for substrate specificity and inhibitor activity. We have also performed a full titration of 18-crown-6 with barium chloride. These results point to future applications for enthalpy array technology including fragment-based screening, secondary assays, and thermodynamic characterization of leads in drug discovery. (c) 2008 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据