期刊
FISH & SHELLFISH IMMUNOLOGY
卷 23, 期 2, 页码 459-472出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2007.01.010
关键词
ESTs; chicadae/profilin; gills; keratinocyte associated protein; oncoprotein nm23; prohibitin; selenoprotein M; white spot syndrome virus (WSSV)
We report the analysis of 872 cDNA clones from a WSSV-infected white shrimp Litopenaeus vannamei gill cDNA library. Comparison against the GenBank protein and nucleotide sequences identified 87% (E <= 10(-2)) as previously known genes, while 13% are novel sequences. The 601 ESTs (87%) represent transcripts of 276 genes. These genes were categorized into 12 groups according to their functions. The more abundant categories were (1) ribosomal proteins (21%), (2) WSSV transcripts and sequences without homology to proteins deposited in the non-redundant database (15%), (3) hypothetical proteins (12%) which include genes never described in shrimp and (4) metabolism related proteins (9%). We also found genes involved in stress and immune response; and only one involved in ion transport. Full-length sequences of keratinocyte associated protein 2 (KCP2), selenoprotein M (SelM), chicadae, prohibitin and oncoprotein nm23 are reported. Their mRNAs steady state levels in addition to ferritin, changed at different times post-WSSV infection as estimated by RT-PCR. These results suggest that WSSV alters gene expression in gills and has led to the identification of novel white shrimp specific genes. (C) 2007 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据