期刊
ANALYTICAL BIOCHEMISTRY
卷 375, 期 2, 页码 370-372出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.01.010
关键词
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We described a novel approach to directly amplify genomic DNA from whole blood and dried blood spotted on filter paper without any DNA isolation by using the PCR buffer with a higher pH, which was optimized as pH 9.1-9.6. Direct PCR on blood treated with various anticoagulants showed that the buffer worked well with the blood treated by citrate, EDTA, or heparinate. DNA fragments with different lengths could be efficiently amplified directly from various forms of blood samples. By coupling the buffer with tetra-PCR, a '' true single-tube genotyping was realized by using whole blood or paper-dried blood as starting material '' (c) 2008 Elsevier Inc. All rights reserved.
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