期刊
MICROCHEMICAL JOURNAL
卷 86, 期 2, 页码 161-165出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2007.02.002
关键词
indole-3-acetic acid; square wave voltammetry; graphite-polyurethane composite electrode; soil sample
A new electroanalytical methodology was developed for the quantification of the phytohormone indole-3-acetic acid (IAA), using a graphite-polyurethane composite electrode (GPU) and the square wave voltammetry (SWV), in 0.1 mol L-1 phosphoric acid solution (PH 1.6). Analytical curves were constructed under optimized conditions (f= 100 s(-1), a= 50 mV, Delta E-i= 5 mV) and the reached detection and quantification limits were 26 mu g L-1 and 0.2 mg L-1, respectively. The developed methodology is simple and accurate for the routine determination of IAA. In order to verify the application of the electroanalytical methodology in fortified soil samples without previous treatment, an IAA assay was performed without serious interferences of the soil constituents. (c) 2007 Elsevier B.V. All rights reserved.
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