期刊
WATER RESEARCH
卷 41, 期 15, 页码 3407-3419出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2007.04.018
关键词
denitrification; diel cycle; microbial mat; nirS and nirK genes; PCR-DGGE; seasonal cycle
Changes in spatio-temporal distribution of bacterial and denitrifying communities were qualitatively studied in a microbial mat from Camargue (France). During a diel and a seasonal cycle, patterns of 16S rRNA and nitrite reductase genes (nirS and nirK) were compared by denaturing gradient gel electrophoresis (DGGE). Statistical analysis of DGGE profiles showed a significant seasonal shift in the community structure of the nirS-containing bacteria with a winter superficial population that extended in summer, whereas the nirK-containing bacteria seemed more affected by vertical gradients rather than by month-to month-changes. Denitrifying activities remained stable during these sampling times. The bacterial community at the surface of the mat also changed according to season, but appeared stable over a day Finally, during a diel cycle nirK populations were localized in zones with large fluctuations of environmental parameters (oxygen, pH, and sulfur levels) while nirS populations seemed more restricted to the permanent anoxic layer of the microbial mat. (C) 2007 Elsevier Ltd. All rights reserved.
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