期刊
ANALYTICAL BIOCHEMISTRY
卷 380, 期 2, 页码 268-275出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.05.045
关键词
protein-imprinted polymer; assistant recognition polymer chains (ARPCs); purification of cellular protein; cloned bacterial protein as template
We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPS) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPS and with only two steps. (c) 2008 Elsevier Inc. All rights reserved.
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