期刊
ANALYTICAL BIOCHEMISTRY
卷 381, 期 2, 页码 254-257出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2008.06.032
关键词
Rayleigh scattering; nonideal solutions; concentrated protein solutions; bovine serum albumin; ovalbumin; ovomucoid
资金
- National Institute of Diabetes and Digestive and Kidney Diseases
A method and apparatus for automated measurement of the concentration dependence of static light scattering Of Protein solutions over a broad range of concentrations is described. The gradient of protein concentrations is created by successive dilutions of an initially concentrated solution contained within the scattering measurement cell, which is maintained at constant total volume. The method is validated by measurement of the concentration dependence of light scattering of bovine serum albumin, ovalbumin, and ovomucoid at concentrations up to 130 g/L. The experimentally obtained concentration dependence of scattering obtained from all three proteins is quantitatively consistent with the assumption that no significant self-association occurs over the measured range of concentrations. Published by Elsevier Inc.
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