期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 407, 期 1, 页码 193-215出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-014-8266-x
关键词
Two-dimensional chromatography; Size-exclusion chromatography; Gel permeation chromatography; Critical chromatography; Gradient chromatography; Complex distributions
Modern polymeric materials are heterogeneous with respect to different structural features, for instance molar mass, composition, and architecture. One-dimensional separation methods such as size-exclusion chromatography (SEC) are insufficient to fully resolve the multidimensional distributions of such complex materials. Therefore, two-dimensional separation methods are increasingly employed to characterize macromolecules. The present article describes in detail the advantages and experimental aspects of two-dimensional macromolecular separations. Selected examples will be discussed to explain the strategies used to separate macromolecules with respect to specific structural features.
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