期刊
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
卷 64, 期 2, 页码 145-150出版社
WILEY
DOI: 10.1111/j.1365-2125.2007.02864.x
关键词
endothelium; ischaemia; reperfusion
What is already known about this subject center dot The production of oxygen free radicals upon administration of nitroglycerin (GTN) and other organic nitrates has been advocated as one of the causes of nitrate-induce tolerance and endothelial dysfunction. center dot It has been shown that nitrates also cause a protective phenomenon that is similar to ischaemic preconditioning, but the mechanisms of this effect have never been investigated in humans. What this study adds center dot We show, in vivo in humans, that GTN causes endothelial protection against ischaemic damage. center dot Furthermore, we show that this effect is mediated by release of oxygen free radicals and by opening of the mitochondrial permeability transition pore. Nitroglycerin (GTN) modulates tissue damage induced by ischaemia and reperfusion (IR) in a mechanism that is similar to ischaemic preconditioning. We set out to study, using a human model of endothelial IR injury, whether GTN-induced endothelial preconditioning is mediated by reactive oxygen species (ROS) formation and/or opening of mitochondrial permeability transition pores (mPTP). In two double-blind, randomized, parallel studies, a total of 66 volunteers underwent measurement of radial artery endothelium-dependent, flow-mediated dilation (FMD) before and after local IR. Subjects were treated, 24 h before IR, with different drugs in order to test the mechanism of GTN-induced endothelial protection. Transdermal GTN (0.6 mg h(-1) for 2 h, administered 24 h before IR) significantly reduced the impairment of FMD caused by IR (placebo group: FMD after IR, 1.3 +/- 0.8%; GTN group: FMD after IR, 5.3 +/- 0.9%, P < 0.01 compared with placebo). This protective effect was lost when vitamin C (2 g i.v. at the time of GTN administration) or ciclosporin (an inhibitor of mPTP, 100 mg 2 h prior to GTN administration) were coadministered (FMD after IR: vit C + GTN group, 2.1 +/- 1.0%; ciclosporin + GTN group, 1.7 +/- 0.8%; both P < 0.05 compared with GTN alone). We demonstrate that GTN protects the endothelium against IR-induced endothelial dysfunction, in an effect similar to delayed ischaemic preconditioning. Using a human model, we provide evidence supporting the concept that this protective effect is mediated by ROS release and mPTP opening upon GTN administration.
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