期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 405, 期 14, 页码 4997-5000出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-6876-3
关键词
Chloramphenicol; Milk; Quantum dots; Lateral flow assay
资金
- Federal Target Program Research and development on priority directions of scientific-technological complex of Russia [16.512.11.2125]
A novel rapid (20 min) fluorescent lateral flow test for chloramphenicol (CAP) detection in milk was developed. The chosen format is a binding-inhibition assay. Water-soluble quantum dots with an emission peak at 625 nm were applied as a label. Milk samples were diluted by 20 % with phosphate buffer to eliminate the matrix effect. The result of the assay could be seen by eye under UV light excitation or registered by a portable power-dependent photometer. The limit of CAP detection by the second approach is 0.2 ng/mL, and the limit of quantitation is 0.3 ng/mL.
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