期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 406, 期 3, 页码 661-667出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-7476-y
关键词
Waterborne virus; Quantification; Droplet digital PCR; Real-time PCR; Digital PCR
资金
- Slovenian Research Agency [L2-4314]
Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR), and needs no standard curve. This new tool is fully amenable for the quantification of viruses in the particularly low concentrations usually found in water samples.
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