Genomic and functional evidence for an ARID 1A tumor suppressor role

ARIDIA is located in 1p36.11, a region frequently deleted in human cancers. Using a novel method to screen for tumorigenic cDNA sequences, we have identified ARIDIA as a presumptive tumor suppressor gene. The transforming ARIDIA sequence was an antisense cDNA, and was the product of a genomic rearrangement, as corroborated in the primary breast carcinoma from which the cDNA had been obtained. In further screening, we identified a lung adenocarcinoma cell line with a highly localized homozygous genomic deletion involving the 5' end of ARID IA. These studies provide strong evidence that ARIDIA is a tumor suppressor gene. (c) 2007 Wiley-Liss, Inc.