期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 405, 期 23, 页码 7541-7544出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-7210-9
关键词
Detection; Chloramphenicol; Lateral flow immunoassay; Europium chelate; Nanoparticle
资金
- Commonweal Scientific Foundation for Industry of Chinese Inspection and Quarantine [201010022]
- Xiamen Scientific Development Program [3502Z20055008]
- National Natural Science Foundation of China [30500454]
- Natural Science Foundation of Fujian Province of China [2007 J0112]
A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is similar to 100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.
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