期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 405, 期 10, 页码 3117-3123出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-013-6743-2
关键词
Cannabis; Delta(9)-Tetrahydrocannabinol; Cloud point extraction; Coacervate; HPLC; Diode array detector; Nonionic surfactant
A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of Delta(9)-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant Dowfax 20B102 was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLC-UV system (diode array detector) with isocratic elution. The parameters and variables affecting the extraction were investigated. Under optimum conditions (1 wt.% Dowfax 20B102, 1 wt.% Na2SO4, T = 318 K, t = 30 min), this method yielded a quite satisfactory recovery rate (similar to 81 %). The limit of detection was 0.04 mu g mL(-1), and the relative standard deviation was less than 2 %. Compared with conventional solid-liquid extraction, this new method avoids the use of volatile organic solvents, therefore is environmentally safer.
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