期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 403, 期 8, 页码 2173-2183出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-012-5840-y
关键词
Molecular imprinting; Protein; Antibody; Recognition sites
资金
- National Basic Research Program of China [2012CB910601]
- National Nature Science Foundation [21005078, 21027002]
- Creative Research Group of the NSFC [21021004]
- Innovation Method Research of MOST [2010IM030500]
- Chinese Academy of Sciences
- State Key Laboratory of Polymer Materials Engineering (Sichuan University) [KF201104]
Protein imprinting is a promising tool for generating artificial biomimetic receptors with antibody-like specific recognition sites. Recently, protein-imprinted materials, as potential antibody substitutes, have attracted much attention in many fields, for example chemical sensors, chromatographic stationary phases, and artificial enzymes, owing to their long-term storage stability, potential re-usability, resistance to harsh environment, and low cost. In this critical review, we focus our discussion on the rational preparation of protein-imprinted materials in terms of choice of template, functional monomer, crosslinker, and polymerization format. In addition, several highlighted applications of protein-imprinted materials are emphasized, not only in well-known fields but also in some unique fields, for example proteomics and tissue engineering. Finally, we propose challenges arising from the intrinsic properties of protein imprinting, for example obtaining the template, heterogeneous binding, and extrinsic competition, for example immobilized aptamers.
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