Sporosarcina koreensis sp nov and Sporosarcina soli sp nov., isolated from soil in Korea

Two Gram-positive, aerobic, spore-forming rods, F73(T) and 180(T), were isolated from upland soil. A phylogenetic analysis of 16S rRNA gene sequences placed both isolates within the genus Sporosarcina, and showed a sequence similarity of 98.9% between the two strains and a similarity of approximately 94.6-97.3% with respect to Sporosarcina species with validly published names. The values for DNA-DNA relatedness between the two isolates and related type strains of the genus Sporosarcina were below 28.0%. For both strains, the major cellular fatty acids were anteiso-C-15:0 and iso-C-15:0. In both cases, the cell-wall peptidoglycan was of the A4 alpha type (L-Lys-D-Glu) and the major menaquinone was MK-7. Diaminopimelic acid was absent from both strains. The genomic DNA G + C contents of strains F73(T) and 180(T) were 46.5 and 44.5 mol%, respectively. On the basis of the phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent two novel species of the genus Sporosarcina, for which the names Sporosarcina koreensis sp. nov. (type strain F73(T) = KACC 11299(T) =DSM 16921(T)) and Sporosarcina soli sp. nov. (type strain 180(T)=KACC 11300(T) =DSM 16920(T)) are proposed.