期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 97, 期 5, 页码 1340-1346出版社
JOHN WILEY & SONS INC
DOI: 10.1002/bit.21360
关键词
microfluidic; artificial liver; biomimetic; hepatocyte; bioreactor
Primary hepatocytes represent a physiologically relevant model for drug toxicity screening. Here, we created a biologically inspired artificial liver sinusoid with a microfluidic endothelial-like barrier having mass transport properties similar to the liver acinus. This unit consisted of a cord of hepatocytes (50 x 30 x 500 mu m) fed by diffusion of nutrients across the microfluidic endothelial-like barrier from a convective transport vessel (10 nL/min). This configuration sustained rat and human hepatocytes for 7 days without an extracellular matrix (ECM) coating. Experiments with the metabolism mediated liver toxicant diclofenac showed no hepatotoxicity after 4 h and an IC50 of 334 +/- 41 mu M after 24 h.
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