期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 142, 期 2, 页码 105-124出版社
HUMANA PRESS INC
DOI: 10.1007/s12010-007-0003-x
关键词
Pichia pastoris; protein expression; methanol induction; dissolved oxygen; gene integration; alcohol oxidase promoter; AOX1
Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.
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