期刊
NEUROCHEMICAL RESEARCH
卷 32, 期 8, 页码 1375-1380出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-007-9317-x
关键词
cytoglobin; oxidative stress; N2a neuroblastoma cell; siRNA; hydrogen peroxide
Cytoglobin (Cygb) is a recently discovered intracellular respiratory globin, which exists in all types of cells. It has been suggested that Cygb has a role in protecting cells against oxidative stress. In the present study we have tested this hypothesis. The N2a neuroblastoma cells were exposed to various kinds of insults, including hydrogen peroxide (H2O2), hypoxia, kainic acid, high extracellular CaCl2, high osmolarity, UV irradiation and heat shock. Among them, only H2O2-treatment induced a significant up-regulation of cytoglobin mRNA level. We stably transfected N2a cells with Cygb-siRNA vectors and successfully knocked down Cygb. The Cygb-siRNA could exacerbate cell death upon H2O2-treatment, as demonstrated by MTT cell viability assay. Thus, Cygb in neuronal cells might be specifically induced under oxidative stress to protect them from death.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据