期刊
JOURNAL OF SEPARATION SCIENCE
卷 30, 期 12, 页码 1870-1874出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200600528
关键词
flavonoids; isoflavonoids; Sophora japonica L.; superose 12
A chromatographic method using 12% cross-linked agarose gel Superose 12 as the separation medium was developed for isolation and purification of the chemical constituents from the pericarp of Sophora japonica L. The mobile phase used for the separation was 2% acetic acid and 7% acetic acid in gradient elution. As a result, eight compounds including four kinds of flavonoids and four kinds of isoflavonoids were obtained in a one-step separation. A straightforward explanation of the separation mechanism of flavonoids and isoflavonoids on Superose 12 is also given. The flavonoids and isoflavonoids are retained on Superose 12 by a combination of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of aglycone and the residues of the cross-linking reagents used in the manufacture of Superose 12.
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