期刊
ACTA PHARMACOLOGICA SINICA
卷 28, 期 8, 页码 1198-1204出版社
ACTA PHARMACOLOGICA SINICA
DOI: 10.1111/j.1745-7254.2007.00602.x
关键词
11 beta-hydroxysteroid dehydrogenase type 1; siRNA; 3T3-L1; cell differentiation; obesity
Aim: To investigate the relationship between 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1), a potential link between obesity and type 2 diabetes, and preadipocyte differentiation. Methods: Mouse 11 beta-HSD1 siRNA plasmids were transfected into 3T3-L1 preadipocytes (a cell line derived from mouse Swiss3T3 cells that were isolated from mouse embryo), for examination of the effect of targeted 11 beta-HSD1 inhibition on differentiation of 3T3-L1 cells. Differentiation was stimulated with 3-isobutyl-1-methyxanthine, insulin, and dexamethasone. The transcription level of the genes was detected by real-time PCR. Results: Lipid accumulation was significantly inhibited in cells transfected with mouse 11 beta-HSD1 siRNA compared with non-transfected 3T3-L1 cells. Fewer lipid droplets were detected in the transfected cells both prior to stimulation and after stimulation with differentiation-inducing reagents. The expression of adipocyte differentiation-associated markers such as lipoprotein lipase and fatty acid synthetase were downregulated in the transfected cells. Similarly, the expression of preadipocyte factor-1, an inhibitor of adipocyte differentiation, was downregulated upon stimulation of differentiation and had no changes in the transfected cells. Conclusion: 11 beta-HSD1 can promote preadipocyte differentiation. Based on this, we propose that 11 beta-HSD1 may be an important candidate mediator of obesity and obesity-induced insulin resistance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据