期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 402, 期 10, 页码 3287-3298出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5624-9
关键词
X-ray fluorescence; Tomography; Speciation; Functional genomics; Plants; Metals
资金
- National Institute of Environmental Health Sciences [P42 ES007373-1, NIEHS P20ES0181702, EPA RD-83459901-1]
- Department of Energy Office of Basic Energy Sciences [DEFG02- 06ER15809]
- US Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-06CH11357]
- Department of Energy (DOE)-Geosciences [DE-FG0292ER14244]
- DOE Office of Biological and Environmental Research, Environmental Remediation Sciences Division [DE-FC09-96-SR18546]
- DOE [DEAC02-98CH10886]
Functional characterisation of the genes regulating metal(loid) homeostasis in plants is a major focus for phytoremediation, crop biofortification and food security research. Recent advances in X-ray focussing optics and fluorescence detection have greatly improved the potential to use synchrotron techniques in plant science research. With use of methods such as micro X-ray fluorescence mapping, micro computed tomography and micro X-ray absorption near edge spectroscopy, metal(loids) can be imaged in vivo in hydrated plant tissues at submicron resolution, and laterally resolved metal(loid) speciation can also be determined under physiologically relevant conditions. This article focuses on the benefits of combining molecular biology and synchrotron-based techniques. By using molecular techniques to probe the location of gene expression and protein production in combination with laterally resolved synchrotron techniques, one can effectively and efficiently assign functional information to specific genes. A review of the state of the art in this field is presented, together with examples as to how synchrotron-based methods can be combined with molecular techniques to facilitate functional characterisation of genes in planta. The article concludes with a summary of the technical challenges still remaining for synchrotron-based hard X-ray plant science research, particularly those relating to subcellular level research.
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