期刊
SMALL RUMINANT RESEARCH
卷 71, 期 1-3, 页码 123-129出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.smallrumres.2006.04.016
关键词
Control Region mtDNA; single nucleotide heteroplasmy; SSCP-DNA sequencing; South American camelids
In the present work, polymerase chain reaction-single strand conformation polymorphism and sequencing were used to investigate the length and nucleotide variability in the Control Region mitochondrial DNA of the four South American camelid species from Argentina. To asses these the complete Control Region of 20 animals, 5 each of guanaco, llama, alpaca and vicunia species were cloned. Seventy-three clones corresponding to the 20 animals were screened and 7 different SSCP patterns were identified. Sequencing of all clones showed 9 different haplotypes contained in the 350 bp hypervariable segment of the Control Region. Interestingly, 3 guanacos, 3 vicunias, 3 alpacas and 1 llama were heteroplasmic for different nucleotide positions. The screening of the Control Region mitochondrial DNA in blood samples from about 200 wild guanacos from Argentine Patagonia supported the above results. After comparison with other vertebrate species, we concluded that nucleotide substitutions are the main cause of heteroplasmy found in Control Region mitochondrial DNA of these taxa. (C) 2006 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据