期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 402, 期 5, 页码 1759-1771出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5318-3
关键词
DNA detection; Gold nanoparticles; PCR-free; Optical detection; Electrochemical detection; SPR; QCM; Scanometric detection
资金
- MIUR [FIRB RBRN07BMCT, RBPR05JH2P ITALNANONET]
A major challenge in the area of DNA detection is the development of rapid methods that do not require polymerase chain reaction (PCR) amplification of the genetic sample. The PCR amplification step increases the cost of the assay, the complexity of the detection, and the quantity of DNA required for the assay. In this context, methods that are able to perform DNA analyses with ultrasensitivity have recently been investigated with the aim of developing new PCR-free detection protocols. Functionalized gold nanoparticles have played a central role in the development of such methods. Here, possibilities offered by functionalized gold nanoparticle in the ultrasensitive detection of DNA are discussed. The different functionalization protocols available for gold nanoparticles and the principal DNA detection methods that are able to detect DNA at the femtomolar to attomolar level are presented.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据