期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 402, 期 1, 页码 381-387出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5395-3
关键词
Surface plasmon resonance; Myelodysplastic syndromes; Vascular endothelial growth factor; Protein markers
资金
- Ministry of Health, Czech Republic [NS10633-3/2009, MZ 02373601]
- Academy of Sciences, Czech Republic
- Baxter, Czech Republic
- [KAN200670701]
The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.
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