期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 401, 期 7, 页码 2069-2078出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5206-x
关键词
Zearalenone; alpha-Zearalenol; Molecularly imprinted polymers; Liquid chromatography; DAD/MS; Endometrial cancer
资金
- Ministry of Science and Higher Education (Warsaw, Poland) [N N204 012038]
This study presents a selective method of isolation of zearalenone (ZON) and its metabolite, alpha-zearalenol (alpha-ZOL), in neoplastically changed human tissue by accelerated solvent and ultrasonic extractions using a mixture of acetonitrile/water (84/16% v/v) as the extraction solvent. Extraction effectiveness was determined through the selection of parameters (composition of the solvent mixture, temperature, pressure, number of cycles) with tissue contamination at the level of nanograms per gram. The produced acetonitrile/water extracts were purified, and analytes were enriched in columns packed with homemade molecularly imprinted polymers. Purified extracts were determined by liquid chromatography (LC) coupled with different detection systems (diode array detection - DAD and mass spectrometry - MS) involving the Ascentis RP-Amide as a stationary phase and gradient elution. The combination of UE-MISPE-LC (ultrasonic extraction - molecularly imprinted solid-phase extraction - liquid chromatography) produced high (R approximate to 95-98%) and repeatable (RSD<3%) recovery values for ZON and alpha-ZOL.
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