期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 401, 期 9, 页码 2805-2810出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5356-x
关键词
Ultra high-performance liquid chromatography; UPLC-MS/MS; 3-hydroxyisovaleric acid; Biotin status indicator; Marginal biotin deficiency
资金
- National Institutes of Health [R37 DK36823, R37 DK36823-26S1, R01 DK79890-01S1]
- Arkansas Biosciences Institute
- National Center for Research Resources [1UL1RR029884]
Measurement of 3-hydroxyisovaleric acid (3HIA) in human urine has been shown to be a useful indicator of biotin status for a variety of clinical situations, including pregnancy. The work described herein presents a novel UPLC-MS/MS method for accurate and precise quantitation of urinary 3HIA. This method utilizes sample preparation prior to quantitation that has been simplified compared to the previous GC-MS method. To demonstrate the suitability of the UPLC-MS/MS method for human bio-monitoring, this method was used to measure 3-HIA in 64 human urine samples from eight healthy adults in whom marginal biotin deficiency had been induced experimentally by egg white feeding. 3HIA was detected in all specimens; the mean concentration [+/- standard deviation (SD)] was 80.6 +/- 51 mu M prior to inducing biotin deficiency. Mean excretion rate for 3HIA (expressed per mol urinary creatinine) before beginning the biotin-deficient diet was 8.5 +/- 3.2 mmol 3HIA per mol creatinine and the mean increased threefold with deficiency. These specimens had been previously analyzed by GC-MS; the two data sets showed strong linear relationship with a correlation coefficient of 0.97. These results provide evidence that this method is suitable for bio-monitoring of biotin status in larger populations.
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