期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 401, 期 3, 页码 883-889出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5128-7
关键词
Carbon nanotubes; Chitosan; Glucose oxidase; Reagentless electrochemical biosensor; AFM
资金
- Comunidad Autonoma de Madrid [S2009/PPQ-1642]
- Ministerio de Ciencia e Innovacion [CTQ2008-05775, FIS2009-12964-C05-04, CSD2007-00010]
The design, characterization and applicability of a nanostructured biosensor platform are described. The biosensor is developed through the immobilization of three components: a polymeric chitosan network previously modified with a redox mediator (denoted as PCF-Pyr-Ch), an enzyme (glucose oxidase, chosen as a model) and carbon nanotubes onto a solid glassy carbon electrode (C). In order to assess the influence of the nanomaterial in the performance of the resulting analytical device, a second biosensor, free of carbon nanotubes, is developed. The characterization of both biosensing platforms was performed in aqueous phosphate buffer solutions using atomic force microscopy technique. In the presence of glucose, both systems exhibit a clear electrocatalytic activity, and glucose could be amperometrically determined at +0.35 V versus Ag/AgCl. The performance of both biosensors was evaluated in terms of sensitivity, detection limit and linear response range. Finally, the enhancement of the analytical response induced by the presence of carbon nanotubes was evaluated.
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