期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 401, 期 7, 页码 2195-2204出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5272-0
关键词
Acetaminophen; ELISA
Acetaminophen antibodies were purified using affinity chromatography and labelled with horseradish peroxidase (HRP). The antibody-HRP conjugate and a new acetaminophen derivative were used in the construction of two immunoassay methods facilitating the direct quantitative measurement of acetaminophen in serum: a 96-well microtitre plate and coated-tube ELISAs. A minimum detection limit of 0.2 mu g mL(-1) and a dynamic range of 0.2 to 10 mu g mL(-1) in serum were achieved using the 96-well microtitre plate ELISA. The tube assay was optimised for the measurement of the clinically critical acetaminophen concentration of 50 to 250 mu g mL(-1) of serum. The quantitative and specific tests could be completed within less than an hour. Common drugs including aspirin showed less than 0.1% cross-reactivity.
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