期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 402, 期 3, 页码 1241-1247出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-011-5508-z
关键词
Ultrasound-enhanced surfactant-assisted dispersive liquid-liquid microextraction; Ketoconazole; Econazole nitrate; High-performance liquid chromatography; Human blood sample
资金
- National Natural Science Foundation of China [30971948]
- China Scholarship Council [2010677504]
A simple and efficient method, based on ultrasound-enhanced surfactant-assisted dispersive liquid-liquid microextraction (UESA-DLLME) followed by high-performance liquid chromatography (HPLC) has been developed for extraction and determination of ketoconazole and econazole nitrate in human blood samples. In this method, a common cationic surfactant, cetyltrimethylammonium bromide (CTAB), was used as dispersant. Chloroform (40 mu L) as extraction solvent was added rapidly to 5 mL blood containing 0.068 mg mL(-1) CTAB. The mixture was then sonicated for 2 min to disperse the organic chloroform phase. After the extraction procedure, the mixture was centrifuged to sediment the organic chloroform phase, which was collected for HPLC analysis. Several conditions, including type and volume of extraction solvent, type and concentration of the surfactant, ultrasound time, extraction temperature, pH, and ionic strength were studied and optimized. Under the optimum conditions, linear calibration curves were obtained in the ranges 4-5000 mu g L-1 for ketoconazole and 8-5000 mu g L-1 for econazole nitrate, with linear correlation coefficients for both > 0.99. The limits of detection (LODs, S/N = 3) and enrichment factors (EFs) were 1.1 and 2.3 mu g L-1, and 129 and 140 for ketoconazole and econazole nitrate, respectively. Reproducibility and recovery were good. The method was successfully applied to the determination of ketoconazole and econazole nitrate in human blood samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据