期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 398, 期 5, 页码 1871-1881出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-4019-7
关键词
Flow cytometry; Microflow cytometer; Hydrodynamic focusing
资金
- NIH [UO1 A1075489]
- ONR [6336]
With a view toward developing a rugged microflow cytometer, a sheath flow system was micromachined in hard plastic (polymethylmethacrylate) for analysis of particles and cells using optical detection. Six optical fibers were incorporated into the interrogation region of the chip, in which hydrodynamic focusing narrowed the core stream to similar to 35 mu m x 40 mu m. The use of a relatively large channel at the inlet as well as in the interrogation region (375 mu m x 125 mu m) successfully minimized the risk of clogging. The device could withstand pressures greater than 100 psi without leaking. Assays using both coded microparticles and cells were demonstrated using the microflow cytometer. Multiplexed immunoassays detected nine different bacteria and toxins using a single mixture of coded microspheres. A549 cancer cells processed with locked nucleic acid probes were evaluated using fluorescence in situ hybridization.
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