期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 45, 期 8, 页码 2359-2364出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00737-07
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资金
- NHLBI NIH HHS [R01 HL083850, HL083850-01] Funding Source: Medline
- NIAID NIH HHS [U01 AI070411, AI056118, U01AI070411, UC1 AI062705, R21 AI055466, U54 AI057158, AI55466, AI51292, R01 AI051292, R21 AI056118, U54AI57158, AI062705] Funding Source: Medline
Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus.
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