期刊
FUNGAL GENETICS AND BIOLOGY
卷 44, 期 8, 页码 726-739出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2006.11.011
关键词
heterothallism; homothallism; Microarray; oomycete; Phytophthora; sexual development
A large-scale screen for genes induced during sexual development was performed in the heterothallic oomycete Phytophthora infestans, the potato blight agent. Of 15,644 unigenes on an Affymetrix chip, 87 were induced > 10-fold during mating, with 28 induced > 100-fold. This was validated in independent matings using RNA blots and RT-PCR. Only 44 genes resembled sequences in GenBank. These encoded regulators such as protein kinases, protein phosphatases, and transcription factors, plus enzymes with metabolic, transport, or cell-cycle activities. Several genes were induced during both mating and asexual sporogenesis, suggesting crosstalk between those pathways. In the homothallic species P. phaseoli, 20% of the 87 genes were expressed at higher levels during conditions conducive to oosporogenesis than non-conducive conditions, while the rest were at similar levels. Many of the latter exhibited higher mRNA concentrations in P. phaseoli than in any non-mating culture of P. infestans, suggesting that part of the sexual pathway is active constitutively in homothallics. (c) 2006 Elsevier Inc. All rights reserved.
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