期刊
BIOPHYSICAL JOURNAL
卷 93, 期 4, 页码 L20-L22出版社
CELL PRESS
DOI: 10.1529/biophysj.107.112375
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资金
- NHLBI NIH HHS [R01 HL054887, HL054887] Funding Source: Medline
- NIGMS NIH HHS [R01 GM069783, GM069783] Funding Source: Medline
Recent studies of several ICK ion-channel blockers suggest that lipid bilayer interactions play a prominent role in their actions. Structural similarities led to the hypothesis that bilayer interactions are important for the entire ICK family. We have tested this hypothesis by performing direct measurements of the free energy of bilayer partitioning (DG) of several peptide blockers using our novel quenching-enhanced fluorescence titration protocol. We show that various ICK peptides demonstrate markedly different modes of interaction with large unilamellar lipid vesicles. The mechanosensitive channel blocker, GsMTx4, and its active diastereomeric analog, D- GsMTx4, bind strongly to both anionic and zwitterionic membranes. One potassium channel gating modi. er, rHpTx2gs, interacts negligibly with both types of vesicles at physiological pH, whereas another, SGTx1, interacts only with anionic lipids. The slope of DG dependence on surface potential is very shallow for both GsMTx4 and D-GsMTx4, indicating complex interplay of their hydrophobic and electrostatic interactions with lipid. In contrast, a cell- volume regulator, GsMTx1, and SGTx1 exhibit a very steep DG dependence on surface potential, resulting in a strong binding only for membranes rich in anionic lipids. The high variability of 5 kcal/ mole in observed DG shows that bilayer partitioning is not a universal property of the ICK peptides interacting with ion channels.
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