Electrochemical simulation of oxidation processes involving nucleic acids monitored with electrospray ionization-mass spectrometry

Oxidation is commonly involved in the alteration of nucleic acids giving rise to diverse effects including mutation, cell death, malignancy, and aging. We demonstrate that electrochemistry represents an efficient and fast method to mimic oxidative modification of nucleic acids occurring in biological systems. Oxidation reactions were performed in a thin-layer cell employing a conductive diamond electrode as the working electrode and were monitored with electrospray ionization-mass spectrometry. Mass voltammograms were acquired for guanosine, adenosine, cytidine, and uridine. The observed oxidation potentials increased in the order guanosine << adenosine