Dendrazawaynes A and B, antifungal polyacetylenes from Dendranthema zawadskii (Asteraceae)

Two new C-14 polyacetylenes dendrazawayne A (7) and dendrazawayne B (9) together with known C-13 polyacetylenes (2, 3), C14 polyacetylenes (1, 4, and 8) and polyacetylene amides (5 and 6) were isolated from the roots of Dendranthema zawadskii. The structures of 7 and 9 were elucidated based on spectroscopic methods including 2D-NMR, HR-TOF-MS, IR, and UV. Compounds 1, 2, 3, 5, and 6 showed moderate activity against tumor cell lines (human small lung cancer cell line A549, melanoma SK-Mel-2, and mouse melanoma 1316171) with IC50 values in the range of 7.4-30 mu g/mL. Compounds 7 and 9, including other polyacetylenes, showed strong activity against the fungus Trichophyton (MIC: 5-10 mu g/mL).