期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 397, 期 8, 页码 3495-3502出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3861-y
关键词
Isotope dilution analysis; Inductively coupled plasma-mass spectrometry; Hyphenated techniques; Quantitative proteomics; Protein labelling
资金
- Spanish MICINN (Ministry for Science and Innovation) [CTQ2008-01725]
ICP-MS techniques based on isotope dilution analysis can be regarded as an emerging tool in quantitative protein analysis. Well-known concepts, for example species-specific and unspecific isotope dilution analysis, which promoted accurate and precise quantification in elemental speciation studies, have nowadays been transferred to the analysis of large biomolecules, e. g. proteins. Besides detection of heteroatom-containing proteins, the artificial introduction of metal-containing labels has attracted much attention and, as a consequence, ICP-MS-based isotope dilution techniques can serve as a valuable quantification tool. In particular, because isotope dilution ICP-MS techniques can enable absolute protein quantification, they can be regarded as an attractive technique in current and prospective proteomics. In this review, recent developments and applications will be highlighted and critically assessed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据