期刊
JOURNAL OF PROTEOME RESEARCH
卷 6, 期 8, 页码 2978-2992出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr0607029
关键词
proteomics; mass spectrometry; LC-MS/MS; pancreas; zymogen granules; acinar cells
资金
- NCRR NIH HHS [S10 RR017990-01] Funding Source: Medline
- NIDDK NIH HHS [R21 DK067283-01A1, R21 DK067283, DK067283, R21 DK067283-02] Funding Source: Medline
The composition of zymogen granules from rat pancreas was determined by LC-MS/MS. Enriched intragranular content, peripheral membrane, and integral membrane protein fractions were analyzed after one-dimensional SDS-PAGE and tryptic digestion of gel slices. A total of 371 proteins was identified with high confidence, including 84 previously identified granule proteins. The 287 remaining proteins included 37 GTP-binding proteins and effectors, 8 tetraspan membrane proteins, and 22 channels and transporters. Seven proteins, pantophysin, cyclic nucleotide phosphodiesterase, carboxypeptidase D, ecto-nucleotide phosphodiesterase 3, aminopeptidase N, ral, and the potassium channel TWIK-2, were confirmed by immunofluorescence microscopy or by immunoblotting to be new zymogen granule membrane proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据