期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 399, 期 10, 页码 3407-3413出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-4390-4
关键词
Click chemistry; High-performance liquid chromatography; Polymer monolithic column; Protein separation
资金
- National Basic Research Program of China [2007CB914100]
- National Natural Science Foundation of China [20935001, 20875050]
- National Natural Science Foundation of Tianjin [10JCZDJC17600]
Two types of macroporous organic polymer monoliths based on glycidyl methacrylate (GMA), 4-vinylbenzyl chloride (VBC) and divinylbenzene (DVB) were prepared inside stainless-steel tubes. Azide functionalities were firstly introduced on the surfaces of poly(GMA-co-DVB) and poly(VBC-co-DVB) monoliths to provide reactive sites for click chemistry. With the application of copper(I)-catalyzed (3 + 2) azide-alkyne cycloaddition, an in-column click-modification approach for covalent attachment of long alkyl chains onto polymer monoliths was developed. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by the scanning electron microscopy, mercury intrusion porosimeter, Fourier transform infrared spectroscopy, and elemental analyses, respectively. The chromatographic performances of the clicked stationary phases were demonstrated with the high separation efficiency for a variety of proteins within 4 min.
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