期刊
BIOPHYSICAL JOURNAL
卷 93, 期 4, 页码 1380-1390出版社
CELL PRESS
DOI: 10.1529/biophysj.107.105007
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资金
- NHLBI NIH HHS [P01 HL041618, HL41618] Funding Source: Medline
- NIDDK NIH HHS [K25 DK071027, DK07678, DK27651, P30 DK027651, T32 DK007678] Funding Source: Medline
Considerable experimental evidence indicates that the primary, nonmotile cilium is a mechanosensory organelle in several epithelial cell types. As the relationship between cellular responses and nature and magnitude of applied forces is not well understood, we have investigated the effects of exposure of monolayers of renal collecting duct chief cells to orbital shaking and quanti. ed the forces incident on cilia. An exposure of 24 h of these cells to orbital shaking resulted in a decrease of amiloridesensitive sodium current by similar to 60% and ciliary length by similar to 30%. The sensitivity of the sodium current to shaking was dependent on intact cilia. The drag force on cilia due to induced fluid. ow during orbital shaking was estimated at maximally 5.2 x 10(-3) pN at 2 Hz, similar to 4 times that of thermal noise. The major structural feature of cilia contributing to their sensitivity appears to be ciliary length. As more than half of the total drag force is exerted on the ciliary cap, one function of the slender stalk may be to expose the cap to greater drag force. Regardless, the findings indicate that the cilium is a mechanosensory organelle with a sensitivity much lower than previously recognized.
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