期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 398, 期 2, 页码 805-813出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-4001-4
关键词
Fluorescent encoded magnetic composite nanoparticles; Multiplex immunoassays equine influenza virus; Equine infectious anemia virus
A new detection format for multiplexed analysis based on fluorescent encoded magnetic composite nanoparticles is presented. Two kinds of virus were analyzed by this new method: equine influenza virus (EIV) and equine infectious anemia virus (EIAV). Firstly, EIV antigen and EIAV antigen were conjugated to two kinds of fluorescent encoded magnetic composite nanoparticles, while the green-emitting CdTe quantum dots (QDs) were attached to the antibody of EIV and EIAV. Then both green-emitting CdTe QD-labeled antibodies and antigens labeled with fluorescent encoded magnetic composite nanoparticles were used to form an immunoassay system for the detection of EIV and EIAV antigens. The method is time-saving and has higher sensitivity (1.3 ng mL(-1) for EIV antigens and 1.2 ng mL(-1) for EIAV antigens) than the conventional methods. A competitive immunoassay method based on this analysis system was used to detect EIV and EIAV antigens in spiked serum samples with satisfactory results.
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