期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 397, 期 8, 页码 3349-3358出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3826-1
关键词
AMPA receptor; Axotomy; Entorhinal cortex; Kainate receptor; Microarray; NMDA receptor; RNA amplification; Transsynaptic degeneration
资金
- NINDS [NS43939]
- NIA [AG09466, AG17617]
- Alzheimer's Association
Custom-designed microarray analysis was utilized to evaluate expression levels of glutamate receptors (GluRs) and GluR-interacting protein genes within isolated dentate gyrus granule cells following axotomy of the principal input, the perforant path (PP). Dentate gyrus granule cells were evaluated by microdissection via laser capture microdissection, terminal continuation RNA amplification, and microarray analysis following unilateral PP transections at seven time points. Expression profiles garnered from granule cells on the side ipsilateral to PP transections were compared and contrasted with naive subjects and mice subjected to unilateral occipital cortex lesions. Selected microarray observations were validated by real-time quantitative PCR analysis. Postlesion time-dependent alterations in specific alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors, kainate receptors, N-methyl-D-aspartate (NMDA) receptors, and GluR-interacting protein genes were found across the time course of the study, suggesting a neuroplasticity response associated with the transsynaptic granule cell alterations following axotomy of incoming PP terminals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据