期刊
MOLECULAR BIOLOGY OF THE CELL
卷 18, 期 8, 页码 2828-2837出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E07-02-0169
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资金
- NCI NIH HHS [P30 CA068485, CA-68485] Funding Source: Medline
- NEI NIH HHS [P30 EY008126, EY-08126] Funding Source: Medline
- NICHD NIH HHS [HD-15052, P30 HD015052] Funding Source: Medline
- NIDDK NIH HHS [P30 DK020593, DK-59637, R01DK-070856, R01 DK070856, R01DK-48370, U24 DK059637, F32DK-072789, DK-58404, R01 DK048370, P30 DK058404, DK-20593, F32 DK072789] Funding Source: Medline
- NIGMS NIH HHS [R01 GM073846, R01 GM073846-02, R01 GM073846-01A1, R01GM-073846, R01 GM073846-03, R01GM-074877] Funding Source: Medline
Cells use multiple pathways to internalize and recycle cell surface components. Although Rablla and Myosin Vb are involved in the recycling of proteins internalized by clathrin-mediated endocytosis, Rab8a has been implicated in nonclathrin-dependent endocytosis and recycling. By yeast two-hybrid assays, we have now demonstrated that Myosin Vb can interact with Rab8a, but not Rab8b. We have confirmed the interaction of Myosin Vb with Rab11a and Rab8a in vivo by using fluorescent resonant energy transfer techniques. Rab8a and Myosin Vb colocalize to a tubular network containing EHD1 and EHD3, which does not contain Rab11a. Myosin Vb tail can cause the accumulation of both Rab11a and Rab8a in collapsed membrane cisternae, whereas dominant-negative Rab11FIP2(129-512) selectively accumulates Rablla but not Rab8a. Additionally, dynamic live cell imaging demonstrates distinct pathways for Rablla and Rab8a vesicle trafficking. These findings indicate that Rab8a and Rab11a define different recycling pathways that both use Myosin Vb.
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