期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 397, 期 3, 页码 1251-1258出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3625-8
关键词
Amino-modified fluorescent magnetic composite nanoparticles; Trace DNA detection; Semiconductor quantum dots
资金
- National Natural Science Foundation of China [20475020, 20075009, 20875036]
- Ministry of Science and Technology of Jilin Province, China [20080544]
A novel, highly sensitive technology for the detection, enrichment, and separation of trace amounts of target DNA was developed on the basis of amino-modified fluorescent magnetic composite nanoparticles (AFMN). In this study, the positively charged amino-modified composite nanoparticles conjugate with the negatively charged capture DNA through electrostatic binding. The optimal combination of AFMN and capture DNA was measured by dynamic light scattering (DLS) and UV-vis absorption spectroscopy. The highly sensitive detection of trace amounts of target DNA was achieved through enrichment by means of AFMN. The detection limit for target DNA is 0.4 pM, which could be further improved by using a more powerful magnet. Because of their different melting temperatures, single-base mismatched target DNA could be separated from perfectly complementary target DNA. In addition, the photoluminescence (PL) signals of perfectly complementary target DNA and single-base mismatched DNA as well as the hybridization kinetics of different concentrations of target DNA at different reaction times have also been studied. Most importantly, the detection, enrichment, and separation ability of AFMN was further verified with milk. Simple and satisfactory results were obtained, which show the great potential in the fields of mutation identification and clinical diagnosis.
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