期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 397, 期 1, 页码 223-231出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-3370-z
关键词
Capillary liquid chromatography-evaporative light-scattering detection; Strata-X cartridges; Heterocyclic aromatic amines; Human urine samples
资金
- Spanish Ministry of Science and Innovation (MICINN)
- JJCC Castilla-La Mancha [CTQ2007-61830, PCC08-00150722]
- INCRECYT
A rapid and simple method for separation and detection of six heterocyclic aromatic amines (2-amino-1-methyl-6-phenylimidazo [4,5-b]-pyridine, 2-amino-1-methyl-imidazo [4,5-f]-quinoline, 2-amino-3,8-dimethyl-imidazo [4,5-f]-quinoxaline, 2-amino-3,7,8-trimethyl-imidazo [4,5-f]-quinoxaline, 2-amino-3,4,8-trimethyl-imidazo [4,5-f]-quinoxaline, and 2-amino-3,4-dimethyl-imidazo [4,5-f]-quinoline) in human urine samples is proposed to reflect daily intake and recent HAAs exposure. This method comprises previous clean-up and preconcentration of the analytes on Strata-X reversed phase extraction cartridges followed by capillary liquid chromatography (CLC) and evaporative light-scattering detection (ELSD). A mobile phase of acetonitrile and ammonium acetate 35 mM at pH 5.15 through a gradient of composition and a flow rate of 15 mu L min(-1) resulted in good separations of the analytes. Temperature and gas pressure were optimized for detection. The CLC-ELSD allows the separation and quantification of HAAs with good resolution, precision, and sensitivity. The usefulness of the proposed method was demonstrated by the analysis of synthetic and natural human urine samples spiked with different concentration levels of heterocyclic amines.
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