期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 397, 期 5, 页码 1861-1872出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-010-3733-5
关键词
Biosensors; DNA modification; Next-generation sequencing; DNA attachment; Bioconjugation; Genomic DNA
资金
- National Institutes of Health [R21 HG004141]
- Center for Advanced Materials and Nanotechnology of Lehigh University
Many biosensors, DNA arrays, and next-generation DNA sequencing technologies need common methods for end modification of random DNA sequences generated from a sample of DNA. Surface immobilization of chemically modified DNA is often the first step in creating appropriate sensing platforms. We describe a simple technique for efficient heterobifunctional modification of arbitrary double-stranded DNA fragments with chosen chemical groups. The modification requires the use of short (10-20 base pairs) synthetic adaptors having desired terminal functional groups and installs known sequences, which can be used for hybridization of primers in the sequencing-by-synthesis approaches. The method, based on ligation under optimized conditions, is selective and provides high yields of the target heterobifunctional DNA product. An additional two-step procedure can be applied to select further for the desired bifunctionalized product using PCR amplification with a chemically modified primer. Both functional groups in the modified DNA are chemically active and can be used in surface immobilization of the DNA strands to create the surface of a biosensor or sequencing chip.
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