期刊
CELL DEATH AND DIFFERENTIATION
卷 14, 期 8, 页码 1433-1442出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.cdd.4402134
关键词
Claspin; caspase cleavage; proteasome; apoptosis; DNA damage checkpoint
Apoptosis plays a crucial role in development and tissue homeostasis. Some key survival pathways, such as DNA damage checkpoints and DNA repair, have been described to be inactivated during apoptosis. Here, we describe the processing of the human checkpoint protein Claspin during apoptosis. We observed cleavage of Claspin into multiple fragments in vivo. In vitro cleavage with caspases 3 and 7 of various fragments of the protein, revealed cut sites near the N- and C- termini of the protein. Using mass spectrometry, we identified a novel caspase cleavage site in Claspin at Asp25. Importantly, in addition to cleavage by caspases, we observed a proteasome- dependent degradation of Claspin under apoptotic conditions, resulting in a reduction of the levels of both full- length Claspin and its cleavage products. This degradation was not dependent upon the DSGxxS phosphodegron motif required for SCF beta-TrCP-mediated ubiquitination of Claspin. Finally, downregulation of Claspin protein levels by short interfering RNA resulted in an increase in apoptotic induction both in the presence and absence of DNA damage. We conclude that Claspin has antiapoptotic activity and is degraded by two different pathways during apoptosis. The resulting disappearance of Claspin from the cells further promotes apoptosis.
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