期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 394, 期 2, 页码 469-479出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2731-y
关键词
Microfluidics/microfabrication; Biosensors; DNA immobilization; Surface functionalization; Bioconjugation; Silane stability
资金
- National Institutes of Health-National Institute of Biomedical Imaging and Bioengineering (NIH-NIBIB) [R21EB007031]
- National Science Foundation [ECS-9876771]
We have evaluated five bioconjugation chemistries for immobilizing DNA onto silicon substrates for microfluidic biosensing applications. Conjugation by organosilanes is compared with linkage by carbonyldiimidazole (CDI) activation of silanol groups and utilization of dendrimers. Chemistries were compared in terms of immobilization and hybridization density, stability under microfluidic flow-induced shear stress, and stability after extended storage in aqueous solutions. Conjugation by dendrimer tether provided the greatest hybridization efficiency; however, conjugation by aminosilane treated with glutaraldehyde yielded the greatest immobilization and hybridization densities, as well as enhanced stability to both shear stress and extended storage in an aqueous environment. Direct linkage by CDI activation provided sufficient immobilization and hybridization density and represents a novel DNA bioconjugation strategy. Although these chemistries were evaluated for use in microfluidic biosensors, the results provide meaningful insight to a number of nanobiotechnology applications for which microfluidic devices require surface biofunctionalization, for example vascular prostheses and implanted devices.
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