期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 394, 期 3, 页码 731-742出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-009-2779-8
关键词
Molecular diagnostics; Real-time PCR; Nucleic acid amplification technique; Microarray; Sequencing; Mass spectrometry; Antibiotic resistance
资金
- German Department for Research and Education (BMBF)
- Robert Bosch Foundation
Molecular diagnostics of infectious diseases, in particular, nucleic-acid-based methods, are the fastest growing field in clinical laboratory diagnostics. These applications are stepwise replacing or complementing culture-based, biochemical, and immunological assays in microbiology laboratories. The first-generation nucleic acid assays were monoparametric such as conventional tests, determining only a single parameter. Improvements and new approaches in technology now open the possibility for the development of multiparameter assays using microarrays, multiplex nucleic acid amplification techniques, or mass spectrometry, while the introduction of closed-tube systems has resulted in rapid microbial diagnostics with a subsequently reduced contamination risk. Whereas the first assays were focused on the detection and identification of microbial pathogens, these new technologies paved the way for the parallel determination of multiple antibiotic resistance determinants or to perform microbial epidemiology and surveillance on a genetic level.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据