期刊
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
卷 154, 期 2, 页码 134-140出版社
ELSEVIER
DOI: 10.1016/j.molbiopara.2007.04.003
关键词
Schistosoma mansoni; single nucleotide polymorphism; computational biology; cathepsin B; comparative modeling
资金
- FIC NIH HHS [D43 TW006580, D43 TW007012, D43 TW007012-04, D43TW006580-05, D43TW007012-03] Funding Source: Medline
Single nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm 14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope. (c) 2007 Elsevier B.V. All rights reserved.
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